Comparative Study on Quantification of Total Catechins Using UV-Vis Spectrophotometric Method and High Performance Liquid Chromatography Techniques

Bioavailability of catechinsin wider range plants was established earlier and it’s utility as medicine against cardiovascular disease, cancer, etc. were also demonstrated. Recent techniques in relation to quantitative analysis total catechins seem be laborious time consuming process handle huge number samples. Established spectrophotometry HPLC methods developed for determination tea extracts compared the present study.UV-Vis spectrophotometric method adopted monitor absorbance at 500 nm reaction mixture (catechins vanillin-H2SO4reagents). Hewlett Packard automated used equipped with Phenomenex Luna 5  phenyl-hexyl column fitted a guard column. Binary elution carried out using Mobile phase A (acetic acid acetonitrile) B (acetonitrile). Method showed good resolution catechin fractions found accurate quantification (sum individual catechins). Results both are comparable variation amongst two ranged between -3.59 2.79% among clones varied seasons.As expected UPASI released exhibited variations their bioavailability. Lean season edge over cropping period sampling terms catechins. obtained from comparable. Two can routine catechins; however, simple, rapid cost effective than that unless composition is warranted.